Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control

Secondary skeletal muscle fiber phenotype is dependent upon depolarization from motor neuron innervation. To study the effects of depolarization on muscle fiber type development, several in vivo and in vitro model systems exist. We have developed a relatively simple-to-use in vitro model system in w...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 798; p. 77
Main Authors Cavanaugh, Eric J, Crew, Jennifer R, DiMario, Joseph X
Format Journal Article
LanguageEnglish
Published United States 2012
Subjects
Cell Separation - methods
Electric Stimulation - methods
Immunohistochemistry - methods
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - physiology
Primary Cell Culture
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Summary:Secondary skeletal muscle fiber phenotype is dependent upon depolarization from motor neuron innervation. To study the effects of depolarization on muscle fiber type development, several in vivo and in vitro model systems exist. We have developed a relatively simple-to-use in vitro model system in which differentiated muscle cells are directly electrically stimulated at precise frequencies. This allows for single cell analysis as well as biochemical and molecular analyses of the mechanisms that control skeletal muscle phenotype.
ISSN:1940-6029
DOI:10.1007/978-1-61779-343-1_5