Genes Upregulated in Human Fetal Membranes by Infection or Labor
OBJECTIVETo determine whether suppression subtractive hybridization can detect genes in fetal membranes that are upregulated by infection, preterm premature rupture of membranes (PROM), or labor. METHODSUsing suppression subtractive hybridization, messenger RNAs from a preterm fetal membrane obtaine...
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Published in | Obstetrics and gynecology (New York. 1953) Vol. 94; no. 3; pp. 441 - 449 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
The American College of Obstetricians and Gynecologists
01.09.1999
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Subjects | |
Online Access | Get full text |
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Summary: | OBJECTIVETo determine whether suppression subtractive hybridization can detect genes in fetal membranes that are upregulated by infection, preterm premature rupture of membranes (PROM), or labor.
METHODSUsing suppression subtractive hybridization, messenger RNAs from a preterm fetal membrane obtained at cesarean delivery without labor (control) were subtracted from a pool of messenger RNAs of three patients with preterm PROM and vaginal delivery. Eight candidate genes identified as upregulated were quantitated by Northern analysis in each of the tissues and in additional patient subgroups.
RESULTSEight differentially upregulated genes were identified in preterm labor with PROM. Four of the genes are known to be involved in the response to inflammation or infection, and subsequent histologic examination showed one of the preterm PROM tissues to be infected. F-actin capping protein and chitinase precursor, not previously known to be involved in infection, were also upregulated in the infected tissue from preterm PROM. Northern blots using additional subgroups of patients showed that a regulatory G-protein signaling protein gene was significantly upregulated at term by labor in addition to significant upregulation of interleukin-8. There was a strong correlation between the gene expression for complement factor-B and duration of membrane rupture in the patients with preterm PROM.
CONCLUSIONTwo novel genes potentially involved in the response to inflammation or infection have been identified. A regulatory G-protein signaling protein and interleukin-8 gene expression were upregulated by labor. Complement factor-B gene expression was directly related to the duration of membrane rupture. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0029-7844 1873-233X |
DOI: | 10.1016/S0029-7844(99)00339-7 |