Characterization of the hrpZ gene from Pseudomonas syringae pv. maculicola M2

Pseudomonas syringae pv. maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy in Pseudomonas syringaepv. maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 medium supplemen...

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Published inBrazilian journal of microbiology Vol. 46; no. 3; pp. 929 - 936
Main Authors Álvarez-Mejía, César, Rodríguez-Ríos, Dalia, Hernández-Guzmán, Gustavo, López-Ramírez, Varinia, Valenzuela-Soto, Humberto, Marsch, Rodolfo
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Sociedade Brasileira de Microbiologia 01.07.2015
Subjects
Arabidopsis - microbiology
Bacterial Outer Membrane Proteins - genetics
Base Sequence
Brassica - microbiology
Culture Media
DNA Transposable Elements - genetics
Genes, Bacterial
MICROBIOLOGY
Mutation - genetics
Plant Diseases - microbiology
Plant Leaves - microbiology
Promoter Regions, Genetic - genetics
Pseudomonas syringae - genetics
Pseudomonas syringae - pathogenicity
transmid
Tn5
mutant non-pathogenic
phylogenetic
hrpZ
Tn 5
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Summary:Pseudomonas syringae pv. maculicola is a natural pathogen of members of the Brassicaceae plant family. Using a transposon-based mutagenesis strategy in Pseudomonas syringaepv. maculicola M2 (PsmM2), we conducted a genetic screen to identify mutants that were capable of growing in M9 medium supplemented with a crude extract from the leaves of Arabidopsis thaliana. A mutant containing a transposon insertion in the hrpZ gene (PsmMut8) was unable to infect adult plants from Arabidopsis thaliana or Brassica oleracea, suggesting a loss of pathogenicity. The promotorless cat reporter present in the gene trap was expressed if PsmMut8 was grown in minimal medium (M9) supplemented with the leaf extract but not if grown in normal rich medium (KB). We conducted phylogenetic analysis using hrpAZB genes, showing the classical 5-clade distribution, and nucleotide diversity analysis, showing the putative position for selective pressure in this operon. Our results indicate that the hrpAZB operon from Pseudomonas syringaepv. maculicola M2 is necessary for its pathogenicity and that its diversity would be under host-mediated diversifying selection.
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ISSN:1678-4405
1678-4405
DOI:10.1590/S1517-838246320140655