Bovine microsatellites: racial differences and association with SINE-elements

Patterns of polymorphism at eight microsatellite loci in three cattle races are described: two large commercial breeds and one endangered landrace. Significant interracial allele frequency differences were found at six loci. The mean heterozygosity was slightly higher in the landrace (H = 0.75) than...

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Bibliographic Details
Published inEXS (Basel) Vol. 67; p. 437
Main Authors Varvio, S L, Kaukinen, J
Format Journal Article
LanguageEnglish
Published Switzerland 1993
Subjects
Alleles
Animals
Base Sequence
Cattle - genetics
DNA Fingerprinting
DNA, Satellite - genetics
Female
Gene Frequency
Male
Molecular Sequence Data
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
Species Specificity
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Summary:Patterns of polymorphism at eight microsatellite loci in three cattle races are described: two large commercial breeds and one endangered landrace. Significant interracial allele frequency differences were found at six loci. The mean heterozygosity was slightly higher in the landrace (H = 0.75) than in the others (H = 0.69). The difference is smaller than that found by DNA-fingerprinting. Intrapopulation distributions of microsatellite allele size (dinucleotide repeat number) were generally bimodal, with certain intermediate repeat types lacking. Sequencing of individual alleles revealed some hidden heterogeneity: an allele defined by PCR-product size actually corresponded to different sequence motifs in different races. Two of the microsatellites occurred as tails of SINE-elements. In contrast to some earlier reports, the position of one of the amplification primers within a high-copy-number SINE-element did not disturb microsatellite amplification; even multiplex PCR was possible.
ISSN:1023-294X
DOI:10.1007/978-3-0348-8583-6_44