LncRNA UCA1 impacts cell proliferation, invasion, and migration of pancreatic cancer through regulating miR-96/FOXO3

• Published in: IUBMB life Vol. 70; no. 4; p. 276
• Main Authors: Zhou, Yongping, Chen, Yigang, Ding, Wenzhou, Hua, Zhiyuan, Wang, Liying, Zhu, Ye, Qian, Haixin, Dai, Tu
• Format: Journal Article
• Language: English
• Published: England 01.04.2018
• Subjects: Apoptosis; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Cell Adhesion; Cell Movement; Cell Proliferation; Forkhead Box Protein O3 - genetics; Forkhead Box Protein O3 - metabolism; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs - genetics; Neoplasm Invasiveness; Neoplasm Metastasis; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Prognosis; RNA, Long Noncoding - genetics; Tumor Cells, Cultured; miR-96; pancreatic cancer; FOXO3; LncRNA UCA1
• ISSN: 1521-6551
• DOI: 10.1002/iub.1699

This study was expected to reveal the regulatory effects of lncRNA UCA1 on pancreatic cancer cell progression through targeting miR-96/FOXO3. Microarray analysis was carried out on 36 cases of pancreatic cancer tissues and 16 cases of adjacent tissues among them. Expression levels of lncRNA UCA1, miR-96, and FOXO3 in pancreatic cancer tissues and cell lines were determined by qRT-PCR. Expression levels of FOXO3 protein were determined by western blot. Cell viability, cell cycle and apoptosis, cell invasion and migration were detected by CCK-8, flow cytometry, and transwell assay, respectively. The colocalization relationship between lncRNA UCA1 and miR-96 was detected by RNA FISH. Whether UCA1 could target miR-96 and whether miR-96 could target FOXO3 3'UTR were verified by dual-luciferase reporter gene assay. High expression of lncRNA UCA1 and FOXO3 and low expression of miR-96 were shown in pancreatic cancer. Inhibition of UCA1 suppressed pancreatic tumor cell proliferation, colony formation, and metastasis, while inhibition of miR-96 promoted pancreatic cancer cell progression. FOXO3 was the downstream target gene of miR-96 and showed the opposite effects. LncRNA UCA1 promoted cell proliferation, invasion, migration and inhibited cell apoptosis of pancreatic cancer through down-regulating miR-96 and up-regulating FOXO3. © 2018 IUBMB Life, 70(4):276-290, 2018.