Polymorphism of Interleukin-6 is not associated with the presence or absence of high HPV E6/E7

• Published in: Anticancer research Vol. 34; no. 7; pp. 3501 - 3504
• Main Authors: Porto, Claudia Regina Cinti, De Oliveira Kleine, João Paulo Ferreira, Longatto Filho, Adhemar, Da Silva, Ismael Dale Cotrim Guerreiro
• Format: Journal Article
• Language: English
• Published: Greece 01.07.2014
• Subjects: Adult; Alleles; Case-Control Studies; Cervix Uteri - virology; Female; Genotype; Human papillomavirus; Humans; Interleukin-6 - genetics; Oncogene Proteins, Viral - biosynthesis; Papillomaviridae - metabolism; Papillomavirus Infections - genetics; Papillomavirus Infections - virology; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Promoter Regions, Genetic; IL6; E6/E7; Gene polymorphism; HPV
• ISSN: 0250-7005; 1791-7530

The present study evaluated the frequency of the polymorphism of Interleukin-6 (IL6) in women positive for E6/E7 Human Papillomavirus (HPV) (n=152) and women negative for HPV (n=238), 390 women in total. Material for analysis was obtained at the Federal University of São Paulo. Interleukin-6 polymorphism was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and analyzed in 3% agarose gel. No significant associations between the frequency of the polymorphism of IL6 in patients expressing E6 and E7 with HPV-positive and -negative reactions were found. There was no statistically significant difference between the case and control group for genotype distribution (p=0.280). Genotypic analysis showed a striking similarity of IL6 polymorphisms in both cases and controls. The allelic distribution in cases and controls for G and C of IL6 were very similar (p=0.186), which could point to similar IL6 functionality for both groups.