PCR quantification of SiO₂ particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes
There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we d...
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| Published in | Chemical communications (Cambridge, England) Vol. 50; no. 73; pp. 10707 - 10709 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
21.09.2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1364-548X 1364-548X |
| DOI | 10.1039/c4cc04480k |
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| Abstract | There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell(-1)). |
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| AbstractList | There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell(-1)). There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb–ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell⁻¹). There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell(-1)).There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell(-1)). |
| Author | Stark, Wendelin J Paunescu, Daniela Eichenseher, Fritz Hoop, Marcus Grass, Robert N Stoessel, Philipp R |
| Author_xml | – sequence: 1 givenname: Marcus surname: Hoop fullname: Hoop, Marcus email: robert.grass@chem.ethz.ch organization: Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zurich, Switzerland. robert.grass@chem.ethz.ch – sequence: 2 givenname: Daniela surname: Paunescu fullname: Paunescu, Daniela – sequence: 3 givenname: Philipp R surname: Stoessel fullname: Stoessel, Philipp R – sequence: 4 givenname: Fritz surname: Eichenseher fullname: Eichenseher, Fritz – sequence: 5 givenname: Wendelin J surname: Stark fullname: Stark, Wendelin J – sequence: 6 givenname: Robert N surname: Grass fullname: Grass, Robert N |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25078035$$D View this record in MEDLINE/PubMed |
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| Snippet | There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to... There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb–ppm range) to... |
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| SubjectTerms | Cell Line, Tumor chemical reactions DNA DNA barcoding DNA Probes - chemistry DNA Probes - metabolism encapsulation Humans Microscopy, Confocal nanoparticles Nanoparticles - analysis Nanoparticles - chemistry Nanoparticles - metabolism Particle Size Polymerase Chain Reaction silica Silicon Dioxide - analysis Silicon Dioxide - chemistry Silicon Dioxide - metabolism toxicology |
| Title | PCR quantification of SiO₂ particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes |
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