PCR quantification of SiO₂ particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we d...

Full description

Saved in:
Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 50; no. 73; pp. 10707 - 10709
Main Authors Hoop, Marcus, Paunescu, Daniela, Stoessel, Philipp R, Eichenseher, Fritz, Stark, Wendelin J, Grass, Robert N
Format Journal Article
LanguageEnglish
Published England 21.09.2014
Subjects
Cell Line, Tumor
chemical reactions
DNA
DNA barcoding
DNA Probes - chemistry
DNA Probes - metabolism
encapsulation
Humans
Microscopy, Confocal
nanoparticles
Nanoparticles - analysis
Nanoparticles - chemistry
Nanoparticles - metabolism
Particle Size
Polymerase Chain Reaction
silica
Silicon Dioxide - analysis
Silicon Dioxide - chemistry
Silicon Dioxide - metabolism
toxicology
Online AccessGet full text
ISSN1364-548X
1364-548X
DOI10.1039/c4cc04480k

Cover

More Information
Summary:There is a strong interest in studying the cellular uptake of silica nanoparticles, particularly at medically relevant concentrations (ppb-ppm range) to understand their toxicology. At present, uptake analysis at these exposure levels is impeded by the high silica background concentration. Here we describe the use of DNA encapsulated within silica particles as a tool to quantify silica nanoparticles in in vitro cell-uptake experiments at low concentrations (down to 10 fg cell(-1)).
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1364-548X
1364-548X
DOI:10.1039/c4cc04480k