Amperometric detection of dopamine in human serum by electrochemical sensor based on gold nanoparticles doped molecularly imprinted polymers

• Published in: Biosensors & bioelectronics Vol. 49; pp. 199 - 203
• Main Authors: Xue, Cheng, Han, Qing, Wang, Yang, Wu, Jinhua, Wen, Tingting, Wang, Ruoyu, Hong, Junli, Zhou, Xuemin, Jiang, Huijun
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier 15.11.2013
• Subjects: Aniline Compounds - chemistry; Biological and medical sciences; Biotechnology; Dopamine - blood; Dopamine Agents - blood; Electrochemical Techniques - methods; Fundamental and applied biological sciences. Psychology; Gold - chemistry; Humans; Limit of Detection; Molecular Imprinting; Nanoparticles - chemistry; Polymers - chemistry; Human; Gold; Dopamine; Nanoparticle; Doping; Polymer; Functionalized AuNPs; Detector; Amperometry; Electrochemistry; Serum; Molecularly imprinted polymers; Detection; Electrochemical sensor; Molecular imprinting
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2013.04.022

In this work, a highly sensitive and selective biomimetic electrochemical sensor for the amperometric detection of trace dopamine (DA) in human serums was achieved by gold nanoparticles (AuNPs) doped molecularly imprinted polymers (MIPs). Functionalized AuNPs (F-AuNPs), a novel functional monomer bearing aniline moieties on the surface of the AuNPs, were prepared via a direct synthesis method and then used to fabricate the conductive MIPs film on the modified electrode by electropolymerization method in the presence of DA and p-aminobenzenethiol (p-ATP). The obtained electrochemical sensor based on the conductive film of AuNPs doped MIPs (AuNPs@MIPs) could effectively minimize the interferences caused by ascorbic acid (AA) and uric acid (UA). The linear range for amperometric detection of DA was from 0.02 μmol L(-1) to 0.54 μmol L(-1) with the detection limit of 7.8 nmol L(-1) (S/N=3). Furthermore, the AuNPs@MIPs modified electrode (AuNPs@MIES) was successfully employed to detect trace DA in different human serums.