Orthotopic Fluorescent Peritoneal Carcinomatosis Model of Esophageal Cancer

• Published in: Anticancer research Vol. 30; no. 10; pp. 3933 - 3938
• Main Authors: GROS, Stephanie J, DOHRMANN, Thorsten, RAWNAQ, Tamina, KURSCHAT, Nina, BOUVET, Michael, WESSELS, Johannes, HOFFMANN, Robert M, IZBICKI, Jakob R, KAIFI, Jussuf T
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.10.2010
• Subjects: Abdomen; Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Animals; Biological and medical sciences; Carcinoma - genetics; Carcinoma - metabolism; Carcinoma - pathology; Cell Line, Tumor; Disease Models, Animal; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Fluorescence; Gastroenterology. Liver. Pancreas. Abdomen; Green Fluorescent Proteins - analysis; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Humans; Medical sciences; Mice; Mice, Nude; Molecular Imaging - methods; Neoplasm Metastasis; Neoplasm Transplantation; Peritoneal Neoplasms - genetics; Peritoneal Neoplasms - metabolism; Peritoneal Neoplasms - pathology; Transfection; Transplantation, Heterologous; Tumors; Orthotopic; in vivo fluorescence imaging; Esophageal disease; Fluorescence; Implantation; Esophagus carcinoma; Orthotopic implantation; Malignant tumor; Metastasis; Esophagus cancer; In vivo; Cancerology; Abdominal disease; Peritoneal cancer; Peritoneal carcinomatosis; carcinomatosis; Digestive diseases; Medical imagery; Models; esophageal carcinoma; Cancer
• ISSN: 0250-7005; 1791-7530

Orthotopic models utilizing orthotopic implantation have been used for developing cancer models of multiple tumor entities. The aim of this study was to evaluate the role of orthotopic injection in establishing a model of esophageal cancer using a human green fluorescent protein (GFP) cell line of human esophageal carcinoma. Nude mice were orthotopically injected in the abdominal esophagus with stably transfected GFP-PT1590 cells. Tumor progression was examined by fluorescence imaging. Fifty percent of animals developed extensive peritoneal spread without a distinct primary tumor at the injection site. Continuous and metastatic spread to the liver, lungs, and lymph nodes was also observed. Fluorescence imaging enabled fast and specific visualization of tumor progression without the need for anesthesia. Intraperitoneal and metastatic tumor spread of GFP-PT1590 esophageal carcinoma demonstrated a highly aggressive but heterogeneous behaviour. Although injection of the esophageal carcinoma cell line GFP-PT1590 did not lead to primary esophageal tumor development at the site of injection, 50% of the mice developed extensive peritoneal spread, as well as lymph node and organ metastasis. The orthotopic cell injection model resulted in peritoneal carcinomatosis of esophageal adenocarcinoma, which could be visualized in real time using fluorescence imaging.