The incidence of rotavirus a isolates of G genotype in Thailand in 2002-2004

Rotaviruses are the leading cause of severe gastroenteritis among infants and young children worldwide. Between November 2002 and March 2004, 36 stool specimens of 108 children with acute diarrhea in Bangkok, Thailand were found positive for Rotavirus A (RV-A) by RT-PCR. The 36 isolates were subject...

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Published inActa virologica (Anglickâa verze) Vol. 49; no. 2; p. 111
Main Authors Theamboonlers, A, Veravigrom, M, Yambangyang, O, Trairatvorakul, P, Chongsrisawat, V, Poovorawan, Y
Format Journal Article
LanguageEnglish
Published Slovakia 2005
Subjects
Antigens, Viral - genetics
Capsid Proteins - genetics
Child
Child, Preschool
Diarrhea - virology
DNA Fingerprinting
Feces - virology
Female
Genotype
Humans
Incidence
Infant
Male
Molecular Epidemiology
Phylogeny
Polymorphism, Restriction Fragment Length
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
Rotavirus - classification
Rotavirus - isolation & purification
Rotavirus Infections - epidemiology
Rotavirus Infections - virology
Sequence Analysis, DNA
Thailand - epidemiology
Thailand
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Summary:Rotaviruses are the leading cause of severe gastroenteritis among infants and young children worldwide. Between November 2002 and March 2004, 36 stool specimens of 108 children with acute diarrhea in Bangkok, Thailand were found positive for Rotavirus A (RV-A) by RT-PCR. The 36 isolates were subjected to genotyping by RFLP analysis and direct sequencing of a part of the gene for major outer capsid glycoprotein VP7. The sequences obtained were subjected to phylogenetic analysis. Among the isolates the genotypes G1 (5.6%), G2 (69.4 %) and G9 (25.0 %) were found. Comparison of these results with those of previous studies covering the period of 1982-1999 revealed a changing pattern of RV-A G genotypes and thus contributed to the understanding of RV-A epidemiology in Thailand. Any vaccine to be developed against this virus should target the G9 genotype as one of common global genotypes.
ISSN:0001-723X