Sister chromatid exchanges and cell proliferative abilities in cultured peripheral blood lymphocytes of patients with rheumatoid and reactive arthritis

• Published in: Clinical and experimental rheumatology Vol. 24; no. 6; pp. 677 - 682
• Main Authors: JARMALAITE, S, MIERAUSKIENE, J, BEITAS, K, RANCEVA, J, LAZUTKA, J. R, BUTRIMIENE, I
• Format: Journal Article
• Language: English
• Published: Pisa Clinical and Experimental Rheumatology 01.11.2006
• Subjects: Acute Disease; Adult; Arthritis, Reactive - blood; Arthritis, Rheumatoid - blood; Biological and medical sciences; Case-Control Studies; Cell Proliferation; Cells, Cultured; Chromosome Aberrations; Chronic Disease; Demography; Diseases of the osteoarticular system; Female; Humans; Inflammatory joint diseases; Interleukin-10 - metabolism; Lymphocyte Activation - genetics; Lymphocytes - physiology; Male; Medical sciences; Middle Aged; Sister Chromatid Exchange - genetics; Human; Immunopathology; Cell culture; Sister chromatid exchange; Diseases of the osteoarticular system; Reactive arthritis; Autoimmune disease; Inflammatory joint disease; Blood; IL-10; Spondylarthropathy; Chronic; Rheumatoid arthritis; Interleukin 10; Lymphocyte; sister chromatid exchanges
• ISSN: 0392-856X; 1593-098X

Analysis of cytogenetic alterations in peripheral blood lymphocytes (PBL) of patients with acute and chronic reactive arthritis (AcReA and ChrReA) and rheumatoid arthritis (RA). The frequencies of sister chromatid exchanges (SCE) and cell proliferative abilities were analysed in PBL from 69 patients with arthritis and 30 healthy controls. The analyses were done on metaphase chromosomes from PBL grown in cell culture for 72 hours. Cytogenetic parameters were compared among study groups and correlations with different clinical, immune and demographic characteristics were analysed. No significant increases in the frequencies of SCE were detected in PBL from patients with AcReA, ChrReA and RA as compared to controls. However, marked impairment of cell proliferative abilities was detected in cultured lymphocytes from patients with arthritis as compared to healthy controls. Significant associations between measures of disease activity and proliferative abilities of PBL were established. Parameters of lymphocyte proliferation were also influenced by concentration of anti-inflammatory cytokine interleukin-10 in the blood of patients. No increased risk of genetic alterations as measured by the rate of SCE was found in patients with RA and ReA. It is most likely that impaired proliferative abilities of peripheral blood lymphocytes are related to disease activity and could reflect systemic changes in cytokines production and intracellular signal transduction.