Immunohistochemical expression of 14-3-3 σ protein in intraductal papillary-mucinous tumor and invasive ductal carcinoma of the pancreas

• Published in: Anticancer research Vol. 26; no. 4B; pp. 3105 - 3110
• Main Authors: OKADA, Toshiyuki, MASUDA, Norihiro, FUKAI, Yasuyuki, SHIMURA, Tatsuo, NISHIDA, Yasuji, HOSOUCHI, Yasuo, KASHIWABARA, Kenji, NAKAJIMA, Takashi, KUWANO, Hiroyuki
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.07.2006
• Subjects: 14-3-3 Proteins; Adenocarcinoma, Mucinous - metabolism; Adenocarcinoma, Mucinous - pathology; Biological and medical sciences; Biomarkers, Tumor - biosynthesis; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - pathology; Carcinoma, Papillary - metabolism; Carcinoma, Papillary - pathology; Exonucleases - biosynthesis; Exoribonucleases; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Immunohistochemistry; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Medical sciences; Neoplasm Proteins - biosynthesis; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Tumors; Immunohistochemistry; Ductal carcinoma; Malignant tumor; Carcinogenesis; Anatomic pathology; 14-3-3 protein; Intraductal papillary-mucinous tumor; Cancerology; pancreatic carcinoma; Digestive diseases; 14-3-3 σ; Intraductal papillary mucinous tumor; Pancreatic disease; Pancreas carcinoma
• ISSN: 0250-7005; 1791-7530

14-3-3 sigma (sigma) has been shown to be overexpressed in pancreatic cancers by a c-DNA microarray technique. However, the expression of 14-3-3 sigma in intraductal papillary-mucinous tumor (IPMT) of the pancreas remains unclear. To evaluate the biological importance of 14-3-3 sigma expression in pancreatic carcinogenesis, immunohistochemistry for 14-3-3 sigma, CDX2, MUC1, MUC2, p53, p16 and Ki-67 was carried out on 33 IPMTs and the results were compared with those for 14 invasive ductal carcinomas (IDCs). The frequency of 14-3-3 sigma immunoreactivity was 70% and 100% in IPMT and IDC, respectively. The frequency of MUCI and Ki-67 immunoreactivity was significantly higher in IDC than IPMT. In IPMT, dark columnar cell types prevailed over clear columnar cell types in terms of the frequency of the Ki-67 labeling index. The overexpression of 14-3-3 sigma was confirmed in both IDC and IPMT. Therefore, this overexpression might occur in the early stages of pancreatic carcinogenesis. Moreover, IPMT composed of dark columnar cells might be a potentially more advanced form than that made up of clear columnar cells.