Role of hepatic neuropeptide Y-Y1 receptors in a methionine-choline-deficient model of non-alcoholic steatohepatitis

• Published in: Life sciences (1973) Vol. 245; pp. 117356 - 9
• Main Authors: Sun, Wei-Wei, ShangGuan, Tao, Zhu, Ping, Li, Hua-Kang, Jiang, Min, Yang, Pan, Li, Lin-Yu, Zhang, Zhi-Hui
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier BV 15.03.2020
• Subjects: Acids; Alanine; Alanine transaminase; Animals; Blotting, Western; Choline; Choline Deficiency - metabolism; Diet; Disease Models, Animal; Fatty liver; Fatty-acid synthase; Fibrosis; Gene expression; High fat diet; Hydrolase; Injuries; Lipids; Lipogenesis; Liver; Liver - metabolism; Liver - pathology; Methionine; Methionine - deficiency; Mice; Mice, Inbred C57BL; Mice, Knockout; Monocyte chemoattractant protein 1; Neuropeptide Y; Nitric oxide; Nitric-oxide synthase; Non-alcoholic Fatty Liver Disease - metabolism; Non-alcoholic Fatty Liver Disease - pathology; Protein biosynthesis; Real-Time Polymerase Chain Reaction; Receptors; Receptors, Neuropeptide Y - metabolism; Rodents; Steatosis; Triglycerides; Triglycerides - metabolism; Tumor necrosis factor-α; Triglyceride hydrolysis; Lipase; Non-alcoholic steatohepatitis; Neuropeptide Y-Y1 receptors
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2020.117356

NPY-Y1R plays an important role in dietary regulation. Although germline knockdown of NPY-Y1R in mice alleviates high-fat-diet-induced obesity and increases CPT1α levels in the liver, the role of the Y1 receptor in specific tissues has not been studied. MCD diet is the most widely used method to establish a model of lean NASH in a short time. We therefore evaluated the role of liver NPY-Y1R in NASH progression. In mice with liver-specific knockout of NPY-Y1R (LivKO) and wild-type control littermates fed MCD diet for 4 weeks, NPY-Y1R deficiency significantly decreased body and liver weight. Moreover, NPY-Y1R deletion protected mice against hepatic steatosis and injury. LivKO decreased TG, TC, and FFA levels in the liver and alanine aminotransferase activity in plasma. To clarify the mechanism, we evaluated the key enzymes involved in triglyceride hydrolase and fatty-acid oxidase. Expression of ATGL, CPT1α, and ACO was significantly increased in LivKO mice, whereas expression of fatty-acid synthase was significantly decreased. mRNA expression analysis revealed a marked reduction of genes involved in de-novo lipogenesis and monosaturated fatty-acid synthesis, including sterol-regulatory element-binding protein 1c and fatty-acid synthase. Moreover, liver injury-related factors were significantly decreased in LivKO mice, such as TNF-α, inducible nitric oxide synthase, and MCP-1. Thus, NPY-Y1R deficiency in the liver alleviates lipid deposition and injury. However, NPY-Y1R did not affect inflammation and fibrosis. NPY-Y1R deficiency in the liver directly suppresses not only hepatic steatosis, but also liver injury, and thus provides a treatment option for NASH.