Pattern of hMLH1, hMSH2 and hMSH6 expression and clinical characteristics in a sample of Malaysian colorectal carcinoma cases

Malignant transformation from normal colonic mucosa to carcinomas may be accelerated by genetic loss or inactivation of genes of the DNA mismatch repair system. The aim of the study was to determine the local incidence and pattern of immunohistochemical expression of mismatch repair proteins namely:...

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Bibliographic Details
Published inMalaysian journal of pathology Vol. 35; no. 1; pp. 45 - 57
Main Authors Khoo, J J, Gunn, A, Peh, S C
Format Journal Article
LanguageEnglish
Published Malaysia College of Pathologists, Academy of Medicine of Malaysia 01.06.2013
Subjects
Adaptor Proteins, Signal Transducing - analysis
Adaptor Proteins, Signal Transducing - biosynthesis
Biomarkers, Tumor - analysis
Carcinoma - metabolism
Carcinoma - mortality
Carcinoma - pathology
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - mortality
Colorectal Neoplasms - pathology
DNA-Binding Proteins - analysis
DNA-Binding Proteins - biosynthesis
Female
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Malaysia
Male
Middle Aged
MutL Protein Homolog 1
MutS Homolog 2 Protein - analysis
MutS Homolog 2 Protein - biosynthesis
Nuclear Proteins - analysis
Nuclear Proteins - biosynthesis
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Summary:Malignant transformation from normal colonic mucosa to carcinomas may be accelerated by genetic loss or inactivation of genes of the DNA mismatch repair system. The aim of the study was to determine the local incidence and pattern of immunohistochemical expression of mismatch repair proteins namely: hMLH1, hMSH2 and hMSH6 in a series of colorectal carcinomas (CRCs) and correlate this to their clinical and pathological features. Forty-three out of 298 cases of CRCs (14.4%) showed abnormal staining pattern for mismatch repair proteins with a majority (65.1%) showing single hMLH1 loss. Tumours with mismatch repair defect (MMR-d) were frequently found at the right side of colon (p<0.001), poorly differentiated carcinomas (p<0.001), produced more mucin (p=0.007), exophytic growth (p=0.007) and were bigger (p=0.002) than tumours with no mismatch repair defect. Immunohistochemical stains for mismatch repair proteins could be done in local laboratories on these selected cases before referring for the expensive molecular test.
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ISSN:0126-8635