Validation of peptide mapping with electrospray mass spectrometry for recombinant proteins of biopharmaceutical interest and its applications as an identity test and a characterization tool

In this paper, the steps required to validate a liquid chromatography peptide mapping method with mass spectrometric detection (LC-MS) for use as an identity test and characterization tool are presented. All aspects of peptide mapping are evaluated and optimized, including protein sample preparation...

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Bibliographic Details
Published inDevelopments in biologicals Vol. 122; p. 29
Main Authors Wei, Z, Tous, G, Yim, A, Hope, J N, Casas-Finet, J R, Folena-Wasserman, G, Schenerman, M A
Format Journal Article
LanguageEnglish
Published Switzerland 2005
Subjects
Peptide Mapping
Pharmaceutical Preparations - chemistry
Recombinant Proteins - chemistry
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
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Summary:In this paper, the steps required to validate a liquid chromatography peptide mapping method with mass spectrometric detection (LC-MS) for use as an identity test and characterization tool are presented. All aspects of peptide mapping are evaluated and optimized, including protein sample preparation (protein reduction, alkylation and enzymatic digestion), high performance liquid chromatography (HPLC) separation of the resulting peptides, and the use of a mass spectrometric detection. In addition, the validation of a single quadruple MS detector is described and the implementation of on-line electrospray ionization MS (ESI-MS) as an adjunct detector to support the investigation of peak differences is presented. Applications of peptide mapping with tandem MS using an electrospray ion-trap instrument throughout the biopharmaceutical product development cycle are discussed, including assessing protein product heterogeneity derived from post-translational modifications (e.g. multiple N- or C-termini, deamidation, oxidation and glycosylation) and protein degradation.
ISSN:1424-6074