Evaluation of Microdissection Testicular Sperm Extraction Results in Patients with Non-Obstructive Azoospermia: Independent Predictive Factors and Best Cutoff Values for Sperm Retrieval

• Published in: Urology journal Vol. 12; no. 6; pp. 2436 - 2443
• Main Authors: Cetinkaya, Mehmet, Onem, Kadir, Zorba, Orhan Unal, Ozkara, Hamdi, Alici, Bulent
• Format: Journal Article
• Language: English
• Published: Iran Urology and Nephrology Research Center 23.12.2015
• Subjects: Adult; Azoospermia - blood; Azoospermia - etiology; Azoospermia - surgery; Chromosome Deletion; Chromosomes, Human, Y; Dissection; Follicle Stimulating Hormone - blood; Humans; Infertility, Male; Inhibins - blood; Karyotype; Klinefelter Syndrome - complications; Luteinizing Hormone - blood; Male; Microsurgery; Organ Size; Predictive Value of Tests; Retrospective Studies; Sex Chromosome Aberrations; Sex Chromosome Disorders of Sex Development - complications; Sperm Retrieval; Testis - pathology; Testosterone - blood; Treatment Outcome
• ISSN: 1735-1308; 1735-546X

Testicular sperm extraction (TESE) for intracytoplasmic sperm injection (ICSI) was first introduced for the treatment of non-obstructive azoospermia. This study was conducted to detect predictive factors affecting the success of microTESE. We retrospectively evaluated the results of 191 cases who underwent microTESE. For each patient, the testicular volume, endocrine profile [follicle stimulating hormone (FSH), luteinizing hormone (LH), free testosterone (FT), total testosterone (TT)], serum inhibin B level, karyotype analysis, and Y chromosome microdeletions were recorded, and all data were analyzed to detect any predictors. The receiver operating characteristic curve, two-sample t-test and regression analysis were used for the statistical analysis. The mean age of the patients was 34.4 ± 5.6 years. Sperm retrieval was successful in 104 (54.5%) patients, and there was no sperm in 87 (45.5%). Seven factors including, testicular size, Johnson score, Y chromosome microdeletion, and serum FSH, LH, FT and TT levels were different between the successful and unsuccessful groups. Six patients had Klinefelter syndrome, and ten patients (5.2%) had a Y chromosome microdeletion (5 AZF-c, 1 AZF-b, 2 AZF-bc, 1 AZF-abc, and 1 AZF-ac). The Johnson score, TT level, family history and Y chromosome microdeletions were determined to be independent predictive factors for sperm found. According to the testicular histology, the sperm-found ratios were 36%, 48.6%, and 95.5% in the sertoli cell only syndrome, maturation arrest, and hypospermatogenesis groups, respectively. According to our results, the Johnson score, TT level, family history-related infertility, and Y chromosome microdeletions were determined to be independent predictive factors for sperm found.