Use of in situ hybridization to examine gene expression in the embryonic, neonatal, and adult urogenital system

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 886; p. 223
• Main Authors: Rumballe, Bree A, Chiu, Han Sheng, Georgas, Kylie M, Little, Melissa H
• Format: Journal Article
• Language: English
• Published: United States 2012
• Subjects: Animals; Gene Expression Profiling - methods; Gene Expression Regulation, Developmental; In Situ Hybridization - methods; Kidney - embryology; Kidney - growth & development; Kidney - metabolism; Mice; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; Urogenital System - embryology; Urogenital System - growth & development; Urogenital System - metabolism

Studies into the molecular basis of morphogenesis frequently begin with investigations into gene expression across time and cell type in that organ. One of the most anatomically informative approaches to such studies is the use of in situ hybridization, either of intact or histologically sectioned t...