Use of in situ hybridization to examine gene expression in the embryonic, neonatal, and adult urogenital system

Studies into the molecular basis of morphogenesis frequently begin with investigations into gene expression across time and cell type in that organ. One of the most anatomically informative approaches to such studies is the use of in situ hybridization, either of intact or histologically sectioned t...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 886; p. 223
Main Authors Rumballe, Bree A, Chiu, Han Sheng, Georgas, Kylie M, Little, Melissa H
Format Journal Article
LanguageEnglish
Published United States 2012
Subjects
Animals
Gene Expression Profiling - methods
Gene Expression Regulation, Developmental
In Situ Hybridization - methods
Kidney - embryology
Kidney - growth & development
Kidney - metabolism
Mice
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
Urogenital System - embryology
Urogenital System - growth & development
Urogenital System - metabolism
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Summary:Studies into the molecular basis of morphogenesis frequently begin with investigations into gene expression across time and cell type in that organ. One of the most anatomically informative approaches to such studies is the use of in situ hybridization, either of intact or histologically sectioned tissues. Here, we describe the optimization of this approach for use in the temporal and spatial analysis of gene expression in the urogenital system, from embryonic development to the postnatal period. The methods described are applicable for high throughput analysis of large gene sets. As such, ISH has become a powerful technique for gene expression profiling and is valuable for the validation of profiling analyses performed using other approaches such as microarrays.
ISSN:1940-6029
DOI:10.1007/978-1-61779-851-1_20