Pulsed diode laser-based monitor for singlet molecular oxygen

Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photosensitizers to be selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Ox...

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Bibliographic Details
Published inJournal of biomedical optics Vol. 13; no. 3; p. 034010
Main Authors Lee, Seonkyung, Zhu, Leyun, Minhaj, Ahmed M, Hinds, Michael F, Vu, Danthu H, Rosen, David I, Davis, Steven J, Hasan, Tayyaba
Format Journal Article
LanguageEnglish
Published United States 01.05.2008
Subjects
Animals
Cell Line, Tumor
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology - instrumentation
Light
Male
Optical Fibers
Oximetry - instrumentation
Prostatic Neoplasms - metabolism
Radiation Dosage
Radiometry - instrumentation
Rats
Singlet Oxygen - analysis
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Summary:Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photosensitizers to be selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in the metastable singlet delta state O2(1Delta) are believed to be the species that destroys cancerous cells during PDT. Monitoring singlet oxygen produced by PDT may lead to more precise and effective PDT treatments. Our approach uses a pulsed diode laser-based monitor with optical fibers and a fast data acquisition system to monitor singlet oxygen during PDT. We present results of in vitro singlet oxygen detection in solutions and in a rat prostate cancer cell line as well as PDT mechanism modeling.
ISSN:1083-3668
DOI:10.1117/1.2927465