In vitro productive infection of non polarised cervical and rectal biopsies by syncytium-inducing and non syncytium inducing primary HIV-1 isolates

An in vitro model was used to study the transmission of HIV-1 primary isolates with different biological phenotype to cervical and rectal non polarised bioptic fragments. The method described allowed the productive infection of both cervical and rectal tissues and the virus produced could be propaga...

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Bibliographic Details
Published inThe New microbiologica Vol. 27; no. 1; p. 71
Main Authors Di Stefano, M, Favia, A, Lisco, A, Caputi Iambrenghi, O, Fiore, J R, Pastore, G
Format Journal Article
LanguageEnglish
Published Italy 01.01.2004
Subjects
Biopsy
Cells, Cultured
Cervix Uteri - cytology
Cervix Uteri - virology
Culture Techniques
Female
Giant Cells - physiology
HIV Infections - transmission
HIV Infections - virology
HIV-1 - pathogenicity
HIV-1 - physiology
Humans
Leukocytes, Mononuclear - virology
Rectum - cytology
Rectum - virology
Virus Replication
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Summary:An in vitro model was used to study the transmission of HIV-1 primary isolates with different biological phenotype to cervical and rectal non polarised bioptic fragments. The method described allowed the productive infection of both cervical and rectal tissues and the virus produced could be propagated onto peripheral blood mononuclear cell cultures. Syncytium-inducing and non-syncytium inducing viral isolates were equally able to produce infection and replication.
ISSN:1121-7138