Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers

The activator of stromelysin 1 gene transcription, SPBP, interacts with the RING finger protein RNF4. Both proteins are ubiquitously expressed and localized in the nucleus. RNF4 facilitates accumulation of specific SPBP-DNA complexes in vitro and acts as a positive cofactor in SPBP-mediated transact...

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Published inThe Journal of biological chemistry Vol. 275; no. 34; pp. 26144 - 26149
Main Authors Lyngsø, C, Bouteiller, G, Damgaard, C K, Ryom, D, Sanchez-Muñoz, S, Nørby, P L, Bonven, B J, Jørgensen, P
Format Journal Article
LanguageEnglish
Published United States 25.08.2000
Subjects
3T3 Cells
Amino Acid Sequence
Animals
Cell Nucleus - metabolism
DNA-Binding Proteins - metabolism
Escherichia coli
Fibroblasts - metabolism
Humans
Matrix Metalloproteinase 3 - genetics
Mice
Molecular Sequence Data
Nuclear Proteins
Peptide Mapping
Protein Binding
RNF4 protein
Saccharomyces cerevisiae
SPBP protein
Transcription Factors - metabolism
Transcription, Genetic
Transcriptional Activation
Ubiquitin-Protein Ligases
Zinc Fingers
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Summary:The activator of stromelysin 1 gene transcription, SPBP, interacts with the RING finger protein RNF4. Both proteins are ubiquitously expressed and localized in the nucleus. RNF4 facilitates accumulation of specific SPBP-DNA complexes in vitro and acts as a positive cofactor in SPBP-mediated transactivation. SPBP harbors an internal zinc finger of the PHD/LAP type. This domain can form intra-chain protein-protein contacts in SPBP resulting in negative modulation of SPBP-RNF4 interaction.
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ISSN:0021-9258
DOI:10.1074/jbc.M003405200