Fluid shear stress suppresses interleukin 8 production by vascular endothelial cells

The effects of shear stress on interleukin 8 (IL-8) production by human umbilical vein endothelial cells (HUVEC) were studied by subjecting the HUVEC to a steady flow laminar shear stress of up to 0.7 N/m(2) in a parallel plate flow chamber. Shear stress decreased IL-8 mRNA expression in a dose and...

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Bibliographic Details
Published inBiorheology (Oxford) Vol. 38; no. 4; p. 347
Main Authors Kato, H, Uchimura, I, Nawa, C, Kawakami, A, Numano, F
Format Journal Article
LanguageEnglish
Published Netherlands 2001
Subjects
Arteriosclerosis - immunology
Cells, Cultured
Culture Media - chemistry
Dose-Response Relationship, Drug
Endothelium, Vascular - immunology
Enzyme Inhibitors - pharmacology
Enzyme-Linked Immunosorbent Assay
Glucose - pharmacology
Humans
Imidazoles - pharmacology
Interleukin-8 - biosynthesis
Interleukin-8 - genetics
Mitogen-Activated Protein Kinases - antagonists & inhibitors
p38 Mitogen-Activated Protein Kinases
Polymerase Chain Reaction - methods
Pyridines - pharmacology
Regional Blood Flow
Rheology
RNA, Messenger - analysis
Stimulation, Chemical
Stress, Mechanical
Time Factors
Umbilical Veins
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Summary:The effects of shear stress on interleukin 8 (IL-8) production by human umbilical vein endothelial cells (HUVEC) were studied by subjecting the HUVEC to a steady flow laminar shear stress of up to 0.7 N/m(2) in a parallel plate flow chamber. Shear stress decreased IL-8 mRNA expression in a dose and time-dependent fashion. High glucose concentrations increased IL-8 mRNA levels in a MAPK-p38-dependent manner, which was suppressed by shear stress. Measurement of IL-8 protein in HUVEC culture media by ELISA demonstrated that IL-8 secretion was also increased by high glucose and suppressed by shear stress. These results suggest that the anti-atherogenic effect of shear stress arises partly from the suppression of the production of IL-8 which has been shown to trigger the adhesion of monocytes to a vascular endothelium and also acts as a mitogen and chemoattractant for vascular smooth muscle cells.
ISSN:0006-355X