Immunohistochemical evidence for sympathetic denervation and reinnervation after necrotic injury in rat myocardium

To study the short and long term effects of myocardial injury on sympathetic nerve fibers, a necrotizing lesion was performed on the diaphragmatic side of rat myocardium by freeze-thawing. Animals were sacrificed at 2, 6, 18, 28 and 105 days after the surgical procedure and paraffin-embedded hearts...

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Published inCellular and molecular biology (Noisy-le-Grand, France) Vol. 41; no. 6; p. 799
Main Authors Nori, S L, Gaudino, M, Alessandrini, F, Bronzetti, E, Santarelli, P
Format Journal Article
LanguageEnglish
Published France 01.09.1995
Subjects
Animals
Biomarkers
Denervation
Freezing
Heart - innervation
Heart Ventricles
Immunohistochemistry - methods
Myocardium - pathology
Necrosis
Nerve Fibers - physiology
Nerve Fibers - ultrastructure
Nerve Regeneration
Rats
Rats, Wistar
S100 Proteins - analysis
Sympathetic Nervous System - physiology
Tyrosine 3-Monooxygenase - analysis
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Summary:To study the short and long term effects of myocardial injury on sympathetic nerve fibers, a necrotizing lesion was performed on the diaphragmatic side of rat myocardium by freeze-thawing. Animals were sacrificed at 2, 6, 18, 28 and 105 days after the surgical procedure and paraffin-embedded hearts were subjected to peroxidase immunohistochemistry. According to previous studies cardiac nerves were visualized by staining their surrounding Schwann cells with an anti-S100 protein antibody. Catecholaminergic axons were specifically identified by an anti-tyrosine hydroxylase antibody. No S-100 positive structures were found in 2-day lesions (denervation). Starting from day 6,S-100 positive structures became progressively more evident (reinnervation) and persisted up to day 105. Many of these newly formed nerve fibers were positive for tyrosine hydroxylase, indicating that a necrotic injury of rat myocardium causes a disappearance of sympathetic innervation which is followed by a phase of sympathetic reinnervation.
ISSN:0145-5680