High-temperature inducible cell-free transcription and replication of double-stranded RNAs within the parasitic protozoan Cryptosporidium parvum

Sporozoites of the protozoan parasite, Cryptosporidium parvum, were found to contain free, full-size plus strands transcribed from two extrachromosomal, cytoplasmic, virus-like double-stranded RNAs (dsRNAs). Cell-free transcription and replication of both dsRNAs were observed in crude sporozoite lys...

Full description

Saved in:
Bibliographic Details
Published inVirology (New York, N.Y.) Vol. 245; no. 2; pp. 331 - 337
Main Authors Khramtsov, N V, Upton, S J
Format Journal Article
LanguageEnglish
Published United States 05.06.1998
Subjects
Animals
Cell-Free System
Cryptosporidium parvum
Cryptosporidium parvum - genetics
Cryptosporidium parvum - virology
RNA, Viral - genetics
Temperature
Transcription, Genetic
Online AccessGet full text

Cover

More Information
Summary:Sporozoites of the protozoan parasite, Cryptosporidium parvum, were found to contain free, full-size plus strands transcribed from two extrachromosomal, cytoplasmic, virus-like double-stranded RNAs (dsRNAs). Cell-free transcription and replication of both dsRNAs were observed in crude sporozoite lysates. RNA polymerase activity was found to be dependent upon addition of Mg2+ or Mn2+, as well as the four ribonucleoside triphosphates, and was insensitive to inhibitors of cellular DNA-dependent RNA polymerase. Semiconservative transcription of the dsRNAs (plus strand synthesis) was observed at a wide range of temperatures, with an optimum of 50 degrees C. In contrast, replication (minus strand synthesis) was detected only at 50 and 60 degrees C.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1998.9181