Effects of recombinant human growth hormone and nandrolone phenylpropionate on the healing of ischemic colon anastomosis in rats

• Published in: Diseases of the colon & rectum Vol. 46; no. 12; pp. 1690 - 1697
• Main Authors: YARIMKAYA, Ali, APAYDIN, Berat, UNAL, Ethem, KARAHICAK, Ilhan, AYDOGAN, T. Fatih, USLU, Ezel, ERGINOZ, Ethem, ARTIS, Tarik, EYUBOGLU, Erhun
• Format: Journal Article
• Language: English
• Published: Secaucus, NJ Springer 01.12.2003
• Subjects: Anastomosis, Surgical; Animals; Biological and medical sciences; Colon - blood supply; Colon - surgery; Disease Models, Animal; Human Growth Hormone - pharmacology; Ischemia - etiology; Ischemia - prevention & control; Male; Medical sciences; Nandrolone - analogs & derivatives; Nandrolone - pharmacology; Rats; Rats, Wistar; Stomach, duodenum, intestine, rectum, anus; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Wound Healing; Antineoplastic agent; Rat; Rodentia; Somatotropin hormone; Surgical anastomosis; Cardiovascular disease; Experimental study; Biological activity; Colonic disease; Vertebrata; Mammalia; Ischemia; Surgery; Animal; Nandrolone; Digestive diseases; Intestinal disease; Colon; Recombinant protein; Anabolic agent; Cicatrization; Comparative study
• ISSN: 0012-3706; 1530-0358
• DOI: 10.1007/BF02660777

Recombinant human growth hormone and nandrolone phenylpropionate are two different anabolic agents. This study was designed to investigate the effects of these anabolic agents on the healing of ischemic colon anastomosis in rats. Seventy adult male Wistar rats were divided into five groups (n = 14). Group I was the sham laparotomy group. In the other groups, surgical procedures consisting of transsection and anastomosis were made at a distance 3 cm from the peritoneal reflection. Group II was the nonischemic control group. Ischemic colon model was produced in the remaining groups. Group III was the untreated control group. Groups IV and V received recombinant human growth hormone and nandrolone phenylpropionate, respectively. Bursting pressure and hydroxyproline levels were measured on the third and seventh postoperative days to evaluate anastomotic healing. Recombinant human growth hormone increased both collagen deposition and bursting pressure significantly at postoperative Days 3 and 7 compared with the sham and untreated control groups (P < 0.005). When compared with the untreated control, nandrolone phenylpropionate significantly increased collagen deposition at postoperative Days 3 and 7 (P < 0.005) and bursting pressure only at postoperative Day 3 (P < 0.005). Recombinant human growth hormone has more favorable therapeutic effects on the healing of ischemic colonic anastomoses than nandrolone phenylpropionate. Recombinant human growth hormone also improves healing of nonischemic colonic anastomosis.