Clonal analysis of a 5-azacytidine-induced specific increase in growth hormone production by rat pituitary cells

The nucleoside analog, 5-azacytidine (5-azaCR) which causes genomic hypomethylation in many cell types, increased GH production by GH4C1 rat pituitary cells 3-5 fold. This increased GH production was independent of changes in prolactin (PRL) synthesis by the same cells; it occurred at 10-fold lower...

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Bibliographic Details
Published inEndocrinology (Philadelphia) Vol. 113; no. 1; p. 418
Main Authors Cherington, P V, Tashjian, Jr, A H
Format Journal Article
LanguageEnglish
Published United States 01.01.1983
Subjects
Animals
Azacitidine - pharmacology
Cell Line
Clone Cells - metabolism
Growth Hormone - biosynthesis
Hydrocortisone - pharmacology
Pituitary Gland - drug effects
Pituitary Gland - metabolism
Rats
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Summary:The nucleoside analog, 5-azacytidine (5-azaCR) which causes genomic hypomethylation in many cell types, increased GH production by GH4C1 rat pituitary cells 3-5 fold. This increased GH production was independent of changes in prolactin (PRL) synthesis by the same cells; it occurred at 10-fold lower 5-azaCR concentrations than those which increased the synthesis of PRL. Elevated GH production persisted for more than 20 population doublings after removal of 5-azaCR from the culture medium, indicating that the induced change was phenotypically stable. GH production remained responsive to stimulation by cortisol in 5-azaCR-treated cells. Clonal analysis demonstrated that the increased GH production was due to a higher incidence of cells in the population which made GH at an enhanced rate.
ISSN:0013-7227
DOI:10.1210/endo-113-1-418