Host defense role of platelets: engulfment of HIV and Staphylococcus aureus occurs in a specific subcellular compartment and is enhanced by platelet activation

• Published in: Blood Vol. 99; no. 11; pp. 4021 - 4029
• Main Authors: YOUSSEFIAN, Tayebeh, DROUIN, Arnaud, MASSE, Jean-Marc, GUICHARD, Josette, CRAMER, Elisabeth M
• Format: Journal Article
• Language: English
• Published: Washington, DC The Americain Society of Hematology 01.06.2002
• Subjects: Biological and medical sciences; Blood coagulation. Blood cells; Blood Platelets - microbiology; Blood Platelets - physiology; Blood Platelets - ultrastructure; Fundamental and applied biological sciences. Psychology; HIV-1 - physiology; Humans; Microscopy, Immunoelectron; Molecular and cellular biology; Phagocytosis; Platelet; Platelet Activation - drug effects; Platelet Activation - physiology; Staphylococcus aureus - physiology; Subcellular Fractions - microbiology; Subcellular Fractions - virology; Thrombin - pharmacology; Human; Retroviridae; Natural immunity; Lentivirus; Defense; Virus; Endocytosis; Hemostasis; Bacteria; Micrococcales; Micrococcaceae; Microorganism; Human immunodeficiency virus; Phagocytosis; Staphylococcus aureus
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood-2001-12-0191

Platelets can bind and phagocytose infectious microorganisms and so enable their transport for a prolonged time. To investigate the subcellular events of these interactions, platelets were incubated either with Staphylococcus aureus or with HIV and analyzed by electron microscopy (EM) and immuno-EM. HIV and bacteria internalization occurred exclusively within platelets showing morphological evidence of activation. Platelet activation enhanced the degree of bacterial internalization. Immunolabeling revealed that the engulfing vacuoles and the open canalicular system (OCS) were composed of distinct antigens. The engulfing vacuoles eventually became the site of prominent alpha-granule release. In platelets incubated with HIV, characteristic endocytic vacuoles were identified close to the plasma membrane, tightly surrounding 1 or 2 HIV particles. Virus particles were also located within the OCS. Immunogold labeling for the viral core protein p24 confirmed the presence of HIV within platelets. Finally, examination of platelets from a patient with acquired immunodeficiency syndrome and high viremia suggested that HIV endocytosis may also occur in vivo.