Chromosome aberrations in Raynaud's phenomenon

We evaluated the occurrence of spontaneous chromosome damage in cultured peripheral lymphocytes of subjects with idiopathic and pre-scleroderma Raynaud's phenomenon, by means of molecular cytogenetic analysis. Using the micronucleus assay as a marker of chromosome alteration, we studied 30 pati...

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Bibliographic Details
Published inEJD. European journal of dermatology Vol. 14; no. 5; p. 327
Main Authors Porciello, Giovanni, Scarpato, Roberto, Storino, Franca, Migliore, Lucia, Ferri, Clodoveo, Cagetti, Francesca, Morozzi, Gabriella, Bellisai, Francesca, Marcolongo, Roberto, Galeazzi, Mauro
Format Journal Article
LanguageEnglish
Published France 01.09.2004
Subjects
Adult
Case-Control Studies
Chromosome Aberrations
Female
Genetic Predisposition to Disease
Humans
In Situ Hybridization, Fluorescence
Micronucleus Tests
Middle Aged
Raynaud Disease - genetics
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Summary:We evaluated the occurrence of spontaneous chromosome damage in cultured peripheral lymphocytes of subjects with idiopathic and pre-scleroderma Raynaud's phenomenon, by means of molecular cytogenetic analysis. Using the micronucleus assay as a marker of chromosome alteration, we studied 30 patients with pre-scleroderma Raynaud's phenomenon, 30 patients with idiopathic Raynaud's phenomenon and 30 healthy subjects. All subjects were classified as ANA-, ACA+ or Scl 70+. To identify the mechanism of micronucleus formation, fluorescence in situ hybridisation analysis was also performed. Pre-scleroderma Raynaud's phenomenon subjects showed significantly higher micronucleus frequencies than idiopathic Raynaud's phenomenon subjects and controls (37.0 +/- 11.5 vs. 11.1 +/- 3.2 and 10.7 +/- 2.7 respectively p < 0.0001). Interestingly, subjects with idiopathic Raynaud's phenomenon displayed micronucleus frequency comparable to that of healthy controls. Furthermore, ACA+ subjects showed the highest micronucleus frequencies (41.0 +/- 7.6) as compared to subjects with Scl 70+ antibody (25.0 +/- 3.5). Our results show that circulating lymphocytes of only pre-scleroderma Raynaud's phenomenon subjects undergo chromosomal damage, as detected by the micronucleus assay, at a higher rate than expected. No prevalence of aneuploidogenic or clastogenic events in micronucleus formation is revealed by fluorescence in situ hybridisation analysis.
ISSN:1167-1122