Plasma neutrophil-activating peptide-1/interleukin-8 and neutrophil elastase in a primate bacteremia model

• Published in: The Journal of infectious diseases Vol. 164; no. 2; pp. 383 - 388
• Main Authors: Redl, H, Schlag, G, Bahrami, S, Schade, U, Ceska, M, Stütz, P
• Format: Journal Article
• Language: English
• Published: United States 01.08.1991
• Subjects: Animals; Biopterins - analogs & derivatives; Biopterins - blood; Disease Models, Animal; Endotoxins - blood; Escherichia coli; Escherichia coli Infections - blood; Escherichia coli Infections - enzymology; Interferon-gamma - blood; Interleukin-1 - blood; Interleukin-6 - blood; Interleukin-8 - blood; Leukocyte Elastase; Male; Neopterin; Pancreatic Elastase - blood; Papio; Papio ursinus; Sepsis - blood; Sepsis - enzymology; Tumor Necrosis Factor-alpha - analysis
• ISSN: 0022-1899
• DOI: 10.1093/infdis/164.2.383

A hyperdynamic sepsis model was set up in seven adult baboons to evaluate neutrophil-activating peptide-1/interleukin (IL)-8 (NAP-1/IL-8), IL-1 beta, IL-6, tumor necrosis factor-alpha (TNF alpha), and IFN-gamma in plasma. By continuous intravenous administration of 10(10) cfu/kg live Escherichia coli over 8 h with additional infusion therapy (less than or equal to 50 ml/kg/h), endotoxin plasma levels of 2.7-22.3 ng/ml were observed. In plasma the kinetics of NAP-1/IL-8 and IL-6 were similar to those of IL-1 at the end of the experiment (8 h) (peak median values, 34, 4197, and 230 ng/ml, respectively). Differences were greatest for IL-6. Monocyte activation during sepsis was confirmed by elevated plasma neopterin levels (91-139 mumol/mmol of creatine). Granulocyte activation was evident from both incipient neutropenia and the massive release of neutrophil elastase into the plasma as measured by a new immunoassay (peak level, 374 ng/ml). Thus, in primate bacteremia, early TNF release is followed by a concomitant increase of NAP-1/IL-8 with plasma kinetics similar to those of IL-6 and IL-1 and accompanied by massive activation of neutrophils.