Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins

Hek is a member of the eph subfamily of receptor tyrosine kinases whose members include elk, hek2, sek, eph and eck among others. Using a soluble form of hek consisting of the extracellular region of the receptor fused to the Fc domain of human IgG1 and an expression cloning strategy, we have isolat...

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Bibliographic Details
Published inOncogene Vol. 10; no. 2; p. 299
Main Authors Kozlosky, C J, Maraskovsky, E, McGrew, J T, VandenBos, T, Teepe, M, Lyman, S D, Srinivasan, S, Fletcher, F A, Gayle, 3rd, R B, Cerretti, D P
Format Journal Article
LanguageEnglish
Published England 19.01.1995
Subjects
Amino Acid Sequence
Base Sequence
DNA, Complementary
Ephrin-A3
Ephrin-A4
Ephrin-B1
Glycosylphosphatidylinositols - metabolism
Ligands
Membrane Proteins - chemistry
Molecular Sequence Data
Proteins - chemistry
Receptor Protein-Tyrosine Kinases - genetics
Receptor, EphB3
Recombinant Proteins - metabolism
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
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Summary:Hek is a member of the eph subfamily of receptor tyrosine kinases whose members include elk, hek2, sek, eph and eck among others. Using a soluble form of hek consisting of the extracellular region of the receptor fused to the Fc domain of human IgG1 and an expression cloning strategy, we have isolated two different but related cDNAs from the human T-lymphoma line HSB-2 that encode ligands for hek. The cDNAs encode proteins of 238 and 201 amino acids (44% amino acid identity) that are anchored to the membrane by glycosylphosphatidylinositol (GPI)-linkage. The proteins encoded by these cDNAs are bound by hek with affinity constants of 2 x 10(8) M-1. These proteins also bind the elk tyrosine kinase receptor. These cDNAs are related to other cDNAs that we have recently isolated from a human placental library that encode ligands for both hek and elk and define an emerging family of ligands for eph-related kinases (LERKs).
ISSN:0950-9232