Lactoferrin-induced up-regulation of zeta (zeta) chain expression in peripheral blood T lymphocytes from cervical cancer patients

Alteration of T-cell-associated signal transduction molecules has recently been implicated in immune suppression in tumour-bearing hosts. Here we report the immunoregulatory effects of human lactoferrin (LF) on zeta-chain expression in peripheral blood T lymphocytes from cervical cancer patients and...

Full description

Saved in:
Bibliographic Details
Published inAnticancer research Vol. 22; no. 3; p. 1897
Main Authors Frydecka, I, Zimecki, M, Bocko, D, Kosmaczewska, A, Teodorowska, R, Ciszak, L, Kruzel, M, Wlodarska-Polinsk, J, Kuliczkowski, K, Kornafel, J
Format Journal Article
LanguageEnglish
Published Greece 01.05.2002
Subjects
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - pharmacology
CD3 Complex - immunology
Female
Flow Cytometry
Humans
Lactoferrin - pharmacology
Membrane Proteins - biosynthesis
Membrane Proteins - immunology
Receptors, Antigen, T-Cell - biosynthesis
Receptors, Antigen, T-Cell - immunology
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Up-Regulation - drug effects
Uterine Cervical Neoplasms - blood
Uterine Cervical Neoplasms - immunology
Uterine Cervical Neoplasms - metabolism
Online AccessGet more information

Cover

More Information
Summary:Alteration of T-cell-associated signal transduction molecules has recently been implicated in immune suppression in tumour-bearing hosts. Here we report the immunoregulatory effects of human lactoferrin (LF) on zeta-chain expression in peripheral blood T lymphocytes from cervical cancer patients and healthy donors. By quantitative flow cytometry analysis, we demonstrated that the mean zeta-chain expression was significantly higher in freshly-isolated T lymphocytes from healthy donors (69%), compared with the patients (38%). Following 3-day culture under standard conditions, zeta-chain expression in T lymphocytes from the patients increased significantly, whereas it dropped in the cells from healthy donors. Anti-CD3 MoAb as well as LF, significantly increased expression of zeta-chain in T cells both from patients and control subjects. The addition of LF to the anti-CD3 MoAb cell cultures resulted in an even higher stimulation of the zeta-chain expression. The results suggest that, in patients with cervical cancer, zeta-chain defects could be corrected by the therapeutic application of LF.
ISSN:0250-7005