Effects of isoflavones from Sophora species on the growth and activation of a mouse macrophage-like cell line

We investigated the effect of eleven isoflavones on the growth and activation of mouse macrophage-like Raw 264.7 cells. The study of structure-activity relationship suggests that both hydrophilic (hydroxyl) and hydrophobic (prenyl) groups within isoflavone molecules are the determinants for the indu...

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Bibliographic Details
Published inAnticancer research Vol. 22; no. 4; p. 2185
Main Authors Tashiro, Miyuki, Suzuki, Fumika, Shirataki, Yoshiaki, Yokote, Yoshiko, Akahane, Kiso, Motohashi, Noboru, Ishihara, Mariko, Satoh, Kazue, Sakagami, Hiroshi
Format Journal Article
LanguageEnglish
Published Greece 01.07.2002
Subjects
Animals
Asparagine - metabolism
Cell Division - drug effects
Cell Line
Cell Survival - drug effects
Citrulline - metabolism
Isoflavones - pharmacology
Lipopolysaccharides - pharmacology
Macrophages - cytology
Macrophages - drug effects
Mice
Nitric Oxide - metabolism
Plant Extracts - pharmacology
Sophora
Structure-Activity Relationship
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Summary:We investigated the effect of eleven isoflavones on the growth and activation of mouse macrophage-like Raw 264.7 cells. The study of structure-activity relationship suggests that both hydrophilic (hydroxyl) and hydrophobic (prenyl) groups within isoflavone molecules are the determinants for the induction of cytotoxic activity. When hydrophobicity was assessed by octanol-water partition coefficient (log P), the maximum cytotoxic activity was observed at a log P value above 2.5. All isoflavones did not significantly stimulate the nitric oxide (NO) production by Raw 264.7 cells, but reduced the NO production by lipopolysaccharide (LPS)-stimulated Raw 264.7 cells, at cytotoxic concentrations. Amino acid analysis in the culture medium demonstrated that isoflavones significantly inhibited the LPS-stimulated production of citrulline and asparagine. Isoflavones inhibited the LPS-stimulated NO production more efficiently than citrulline and asparagine production, possibly due to their NO scavenging activity. These data suggest that the inhibiton of LPS action by isoflavones may be coupled with their cytotoxic activity.
ISSN:0250-7005