A new approach to cancer therapy due to appropriate uptake and retention kinetics of meta-tetrahydroxy-phenylchlorin in a human fibroblast cell line

Studies have shown that meta-tetrahydroxy-phenylchlorin is an efficient tumor targeting agent for laser photodynamic therapy. The effectiveness of this approach for cancer treatment depends on drug concentration, incubation time and extracellular protein. We studied uptake and retention kinetics of...

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Bibliographic Details
Published inCancer biochemistry biophysics Vol. 15; no. 3; p. 171
Main Authors Wierrani, F, Fiedler, D, Schnitzhofer, G, Stewart, J C, Gharehbaghi, K, Henry, M, Grin, W, Grünberger, W, Krammer, B
Format Journal Article
LanguageEnglish
Published England 01.04.1996
Subjects
Antineoplastic Agents - administration & dosage
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacokinetics
Cell Line
Drug Stability
Fibroblasts - cytology
Fibroblasts - metabolism
Humans
Methyldopa - administration & dosage
Methyldopa - analogs & derivatives
Methyldopa - chemistry
Methyldopa - pharmacokinetics
Photochemotherapy - methods
Radiation-Sensitizing Agents - administration & dosage
Radiation-Sensitizing Agents - chemistry
Radiation-Sensitizing Agents - pharmacokinetics
Sensitivity and Specificity
Skin - cytology
Skin - metabolism
Solutions
Spectrometry, Fluorescence
Temperature
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Summary:Studies have shown that meta-tetrahydroxy-phenylchlorin is an efficient tumor targeting agent for laser photodynamic therapy. The effectiveness of this approach for cancer treatment depends on drug concentration, incubation time and extracellular protein. We studied uptake and retention kinetics of mTHPC in a human fibroblast cell line. Our results clearly demonstrate a difference in the amount of extracellular mTHPC at an incubation temperature of 37 degrees C compared to 20 degrees C and 4 degrees C. pH-values were always constant and not responsible for the increase. Furthermore, both absorption and fluorescence of mTHPC increase when incubated at normal human body temperature. Incubation of human fibroblast cells with mTHPC (10 micg/mL) showed that intracellular mTHPC increases in a linear manner reaching saturation after 24 hours and declining until 48 hours with concommitant increase of supernatant mTHPC. Therefore, we believe that tumor cells can be treated optimally with PDT following a delay > 24 hours after drug administration with a minimum of damage to surrounding normal tissues.
ISSN:0305-7232