Column-switching high-performance liquid chromatographic (HPLC) determination of hydrochlorothiazide in rat, dog, and human plasma

A fully automated HPLC assay for hydrochlorothiazide in plasma has been developed using a column-switching technique. The method involves direct injection of plasma to the extraction column for sample cleanup followed by switching onto the analytical column. Good precision, accuracy, and linearity w...

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Bibliographic Details
Published inPharmaceutical research Vol. 7; no. 12; p. 1257
Main Authors Kuo, B S, Mandagere, A, Osborne, D R, Hwang, K K
Format Journal Article
LanguageEnglish
Published United States 01.12.1990
Subjects
Animals
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - standards
Dogs
Evaluation Studies as Topic
Humans
Hydrochlorothiazide - blood
Rats
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Summary:A fully automated HPLC assay for hydrochlorothiazide in plasma has been developed using a column-switching technique. The method involves direct injection of plasma to the extraction column for sample cleanup followed by switching onto the analytical column. Good precision, accuracy, and linearity were obtained over a range of 25 to 2000 ng/ml in rat, dog, and human plasma. The column-switching method has also been validated by comparison with a conventional HPLC method requiring a cumbersome plasma extraction procedure. Since the method is simple, rapid, and reproducible, it is useful for determination of hydrochlorothiazide levels in animal and human plasma.
ISSN:0724-8741
DOI:10.1023/A:1015933820971