Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine

• Published in: Animal reproduction Vol. 21; no. 3; p. e20240064
• Main Authors: Fiorenza, Mariani Farias, Bridi, Alessandra, Dos Santos, Gislaine, Rosa, Paola Maria, Alves, Luana, Ferst, Juliana Germano, Ferraz, Priscila Assis, Pugliesi, Guilherme, Pohler, Ky, Perecin, Felipe, Meirelles, Flávio Vieira, da Silveira, Juliano Coelho
• Format: Journal Article
• Language: English
• Published: Brazil Colégio Brasileiro de Reprodução Animal 01.01.2024
• Subjects: BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR); migration; bovine; nanoparticles; uterus; neutrophils
• ISSN: 1984-3143; 1806-9614; 1984-3143
• DOI: 10.1590/1984-3143-AR2024-0064

This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils and . For the experiment, six Nellore heifers ( ) received an intrauterine infusion seven days after ovulation with 1X PBS only (sham group; n=1), 1X PBS stained with lipophilic dye PKH26 (control group; n=2), or FF-derived EVs stained with PKH26 (treated group; n=3). Plasma was collected at 0, 10, 30, 60-, 180-, 360-, 720-, and 1440-min post-infusion to obtained EVs for analysis by nano flow cytometry. Labeled EVs were present in the bloodstream at 30- and 60-min post-infusion in the treatment group. Additionally, plasma derived-EVs from all groups were positive for Calcein-AM, Alix, Syntenin, and Calnexin, which confirm the presence of EVs. The second experiment utilized the plasma-derived EVs from the heifers from 30 and 60 min timepoints to evaluate if neutrophils can uptake EVs . As results, it was possible to observe the presence of labeled EVs in neutrophils treated with plasma derived-EVs from the treatment group. In summary, our results suggest that labeled EVs can migrate from the uterine environment rapidly and interact with circulating immune cells in bovine.