Production of alpha-interferon by human leukocytes

We examined the capacity of human leukocytes to produce alpha-interferon subtypes following viral induction. It was shown that the beta-propiolactone inactivated viral inductor stimulated the production of a complete set of native alpha-interferon subtypes. Acid treatment of native interferon at pH...

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Bibliographic Details
Published inActa virologica (Anglickâa verze) Vol. 34; no. 6; p. 529
Main Authors Scheglovitova, O N, Kulieva, A M, Orlova, T G
Format Journal Article
LanguageEnglish
Published Slovakia 01.12.1990
Subjects
Humans
Hydrogen-Ion Concentration
Interferon Type I - biosynthesis
Interferon Type I - blood
Leukocytes - metabolism
Leukocytes - microbiology
Orthomyxoviridae - growth & development
Propiolactone - pharmacology
Virus Activation
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Summary:We examined the capacity of human leukocytes to produce alpha-interferon subtypes following viral induction. It was shown that the beta-propiolactone inactivated viral inductor stimulated the production of a complete set of native alpha-interferon subtypes. Acid treatment of native interferon at pH 2.0 inactivated the acid-labile portion of alpha-interferon. Its exposure to 37 degrees C for 5 days and repeated acid treatment at pH 2.0 resulted in additional inactivation of some interferon pool fractions. The acid-labile subtypes of human alpha-interferon were formed in a nonadherent mononuclear cell fraction.
ISSN:0001-723X