Use of eluates of filter paper blood spots in ELISA for the serodiagnosis of leprosy

Blood samples were collected from 59 leprosy patients and 35 normal healthy subjects by veni-puncture and finger prick methods to obtain serum samples and blood spots on filter paper respectively. The serum samples at 1:300 dilutions and the eluates of dried blood spots at 1:40, 1:80, 1:160 and 1:32...

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Published inIndian journal of medical research (New Delhi, India : 1994) Vol. 89; pp. 150 - 157
Main Authors Dhandayuthapani, S, Anandan, D, Vasanthi, B, Bhatia, V N
Format Journal Article
LanguageEnglish
Published India 01.05.1989
Subjects
Blood Specimen Collection - methods
Disaccharides
Enzyme-Linked Immunosorbent Assay - methods
False Negative Reactions
Glycolipids - immunology
Humans
Leprosy - diagnosis
Leprosy - immunology
Serologic Tests
Serum Albumin, Bovine - immunology
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Summary:Blood samples were collected from 59 leprosy patients and 35 normal healthy subjects by veni-puncture and finger prick methods to obtain serum samples and blood spots on filter paper respectively. The serum samples at 1:300 dilutions and the eluates of dried blood spots at 1:40, 1:80, 1:160 and 1:320 dilutions were applied in ELISA to measure the antibody levels (IgM) against synthetic ND-O-BSA antigen. The antibody levels were found to be high in the multibacillary leprosy patients than the pauci-bacillary patients irrespective of whether serum samples or eluates were used. The OD values obtained at 1:160 dilution of the eluates were equivalent to that of values obtained at 1:300 dilution of the serum samples. The positivities differ in different dilution of the eluates, showing the highest in the 1:40 dilution and the lowest in the 1:320 dilution.
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ISSN:0971-5916