Clinical significance of IgG anti-Sm antibodies in patients with systemic lupus erythematosus

• Published in: Journal of rheumatology Vol. 17; no. 4; p. 469
• Main Authors: Yasuma, M, Takasaki, Y, Matsumoto, K, Kodama, A, Hashimoto, H, Hirose, S
• Format: Journal Article
• Language: English
• Published: Canada 01.04.1990
• Subjects: Adolescent; Adult; Aged; Antibodies, Antinuclear - analysis; Antibody Specificity; Autoantigens - immunology; Autoantigens - isolation & purification; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G - immunology; Lupus Erythematosus, Systemic - immunology; Lupus Erythematosus, Systemic - physiopathology; Male; Middle Aged; Ribonucleoproteins - immunology; Ribonucleoproteins, Small Nuclear; snRNP Core Proteins

Free Sm was purified by gel filtration and anti-Sm affinity chromatography. Using this purified antigen, an ELISA for anti-Sm was performed. Three hundred and fifty patients with various rheumatic diseases were studied with respect to immunoglobulin classes of anti-Sm by ELISA. A high frequency of I...