Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus

• Published in: Journal of rheumatology Vol. 26; no. 8; p. 1715
• Main Authors: Satoh, A, Suzuki, K, Takayama, E, Kojima, K, Hidaka, T, Kawakami, M, Matsumoto, I, Ohsuzu, F
• Format: Journal Article
• Language: English
• Published: Canada 01.08.1999
• Subjects: Adolescent; Adult; Annexin A4 - immunology; Annexin A5 - immunology; Antiphospholipid Syndrome - blood; Antiphospholipid Syndrome - immunology; Autoantibodies - analysis; Autoantibodies - blood; beta 2-Glycoprotein I; Binding, Competitive - drug effects; Cardiolipins - immunology; Female; Glycoproteins - immunology; Humans; Lupus Erythematosus, Systemic - blood; Lupus Erythematosus, Systemic - immunology; Male; Middle Aged; Phosphatidylserines - pharmacology
• ISSN: 0315-162X

Annexins (Anx) are a family of structurally related proteins that bind to phospholipids in a calcium dependent manner. It has been reported that antibodies to Anx V, which acts as an antithrombotic protein, are associated with thrombosis in systemic lupus erythematosus (SLE) and/or antiphospholipid syndrome (APS). Homology between the primary structures of Anx IV and Anx V is the highest among members of the Anx family. We investigated whether anti-Anx IV autoantibodies can be detected in the sera of patients with SLE and/or APS. Seventy-four patients with SLE/APS were divided into 3 groups: Group A, patients with SLE but no clinical or serological features of APS; Group B, patients with SLE having only serological signs of APS; and Group C, patients with clinical symptoms and serological signs of APS. Anx IV and Anx V were prepared by recombinant technique. Anti-Anx IV, Anx V, cardiolipin (CL), and CL beta2-glycoprotein I were detected by ELISA. Anti-Anx IV was found in 15.4% of Group A, 20.0% of Group B, and 21.7% of Group C. Anti-Anx V was found in 3.8% of Group A, 28.0% of Group B, and 30.4% of Group C. Significant correlations were noted between anti-Anx IV titer and anti-Anx V titer (p<0.001), and between anti-Anx IV titer and aCL titer (p<0.01). Anti-Anx IV and V antibodies were characterized in the sera of patients with SLE/APS. Significantly higher frequency of arterial or venous thrombosis was found in patients with anti-Anx V.