Expression of calretinin in human mesothelioma cell lines and cell cycle analysis by flow cytometry

Human mesothelioma cell lines were studied concerning the expression of the calcium-binding protein calretinin (CR), and the relation of the DNA index to their cell cycle. The results obtained for cell lines with different morphological characteristics, were compared to those from human mesothelial...

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Bibliographic Details
Published inAnticancer research Vol. 21; no. 1A; p. 181
Main Authors Saydan, N, Salicio, V, Cappelli-Gotzos, B, Gotzos, V
Format Journal Article
LanguageEnglish
Published Greece 01.01.2001
Subjects
Blotting, Western
Calbindin 2
Cell Cycle
Cell Line, Transformed
Cell Nucleus - genetics
Cell Size
Cell Transformation, Viral
DNA, Neoplasm - analysis
Flow Cytometry
Humans
Immunohistochemistry
Mesothelioma - genetics
Mesothelioma - metabolism
Mesothelioma - pathology
Mitotic Index
Phenotype
S100 Calcium Binding Protein G - immunology
S100 Calcium Binding Protein G - metabolism
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Summary:Human mesothelioma cell lines were studied concerning the expression of the calcium-binding protein calretinin (CR), and the relation of the DNA index to their cell cycle. The results obtained for cell lines with different morphological characteristics, were compared to those from human mesothelial cells transfected with SV40 to escape senescence. Immunocytochemical expression of calretinin (confirmed by immunoblot) was observed in all mesothelioma cell lines but not in the control cells. It is suggested that calretinin is active in the first steps of carcinogenesis in all human mesotheliomas and during several stages in the evolution towards malignancy.
ISSN:0250-7005
1791-7530