Expression of T-lineage early developmental markers by cells establishing atopic dermatitis skin infiltrates

The association between the atopic dermatitis, eczema and T-cell immunodeficiency disorders are well known, thus suggesting that bone marrow T-precursors could use the micro-environment of the skin as an extrathymic site for compensatory ontogenesis. In keeping with this hypothesis, we analyzed the...

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Bibliographic Details
Published inJournal of investigational allergology & clinical immunology Vol. 8; no. 6; p. 359
Main Authors Mastrandrea, F, Cadario, G, Bedello, P G, Nicotra, M R, Natali, P G
Format Journal Article
LanguageEnglish
Published Spain 01.11.1998
Subjects
Adult
Animals
Antibodies, Monoclonal - immunology
Antigens, CD - analysis
Biomarkers
Cell Differentiation
Dermatitis, Atopic - immunology
Dermatitis, Atopic - pathology
DNA Nucleotidylexotransferase - metabolism
Humans
Immunophenotyping
Mice
Skin - immunology
Skin - pathology
T-Lymphocyte Subsets - immunology
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Summary:The association between the atopic dermatitis, eczema and T-cell immunodeficiency disorders are well known, thus suggesting that bone marrow T-precursors could use the micro-environment of the skin as an extrathymic site for compensatory ontogenesis. In keeping with this hypothesis, we analyzed the atopic dermatitis skin lymphocytic infiltrate phenotypes to establish their ontogenetic stage of development. Cryostatic sections (4 microns) obtained from acute lesional skin biopsies of six patients with extrinsic atopic dermatitis were processed with indirect immunoperoxidase, using a panel of first-step monoclonal antibodies (mAb) specific to CD104 (integrin beta 4 chain), CD90w (Thy 1 antigen), CD44 (phagocytic glycoprotein-1; Pgp-1), CD1a and the DNA polymerase terminal deoxynucleotidyl transferase (TdT). Within the lymphocytic dermal infiltrate different levels of immunoreactivity were observed with respect to CD104, CD90w and CD1a. A strong, spread staining was also detected for mAb specific to Pgp-1 and TdT. Together, the reported features indicate that the atopic dermatitis skin-homing lymphocytes express immunophenotypes which are distinctive of the early T-ontogeny.
ISSN:1018-9068