Frequency analysis of cytotoxic T lymphocyte precursors in search for donors in bone marrow transplantation

The usefulness of cytotoxic T lymphocytes precursors (CTLp) frequency analysis in the search for donors in bone marrow transplantation was studied. The frequency of anti-recipient CTLp was approached by limiting dilution assay in HLA matched unrelated, HLA partially matched related and HLA genotypic...

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Published inNeoplasma Vol. 42; no. 1; pp. 3 - 8
Main Authors CUKROVA, V, DOLEZALOVA, L, LOUDOVA, M, MANTEJKOVA, E, KORINKOVA, P, LUKASOVA, M, STARY, J
Format Journal Article
LanguageEnglish
Published Bratislava Slovak Academic Press 1995
Subjects
Biological and medical sciences
Bone Marrow - immunology
Bone Marrow Cells
Bone Marrow Transplantation
Cytotoxicity Tests, Immunologic
Female
Graft vs Host Disease - immunology
HLA Antigens - immunology
Humans
Immunity, Cellular
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
T-Lymphocytes, Cytotoxic - immunology
Tissue Donors
Human
HLA-System
Histocompatibility system
Surgery
T-Lymphocyte
Bone marrow
Cytotoxicity
Exploration
Transplantation
Homotransplantation
Precursor
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Summary:The usefulness of cytotoxic T lymphocytes precursors (CTLp) frequency analysis in the search for donors in bone marrow transplantation was studied. The frequency of anti-recipient CTLp was approached by limiting dilution assay in HLA matched unrelated, HLA partially matched related and HLA genotypically identical donors. The majority of patients examined were affected with different hematological malignancies. Alloreactive CTLp recognizing non-HLA gene products were not detected in pretransplant examination of two pairs of HLA identical siblings. However, an increased incidence of allospecific CTLp was identified in HLA matched MLC negative unrelated pairs. Thus, CTLp assay allowed to uncover the residual Class I incompatibilities that remained hidden in standard serotyping. In two matched unrelated pairs with high pretransplant CTLp frequency the severe acute graft-versus-host disease (GVHD) developed after bone marrow transplantation. Examination of other relatives in patients lacking an HLA identical sibling showed the importance of Class I incompatibility for CTLp generation as well. The lack of correlation between CTLp frequency and HLA-D disparity could suggest that Class II antigens do not participate in CTLp induction. With one exception we had good correlation between MLC and DNA analysis of Class II antigens demonstrating that MLC gives interpretable results even in unrelated pairs. Our results demonstrate the significance of CTLp frequency assay in detection of residual Class I incompatibilities in matched unrelated pairs and in assessment of Class I compatibility in related pairs. For that it should be used in the final selection of BMT donors.
ISSN:0028-2685