Retroviruses that express different ras mutants cause different types of tumors in chickens

We have used replication-competent retroviral vectors to express avian and murine ras genes in cultured chick embryo fibroblasts (CEF) and in chickens. Since the viral vectors are identical, it is possible to compare the oncogenic potential of the ras genes directly. The normal (12 gly) form of chic...

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Bibliographic Details
Published inOncogene Vol. 7; no. 1; p. 141
Main Authors Givol, I, Greenhouse, J J, Hughes, S H, Ewert, D L
Format Journal Article
LanguageEnglish
Published England 01.01.1992
Subjects
Animals
Bone Neoplasms - genetics
Bone Neoplasms - microbiology
Cell Transformation, Viral - genetics
Chickens
Genes, ras - genetics
Lung Neoplasms - genetics
Lung Neoplasms - microbiology
Muscular Diseases - genetics
Muscular Diseases - microbiology
Mutation - genetics
Neoplasms, Experimental - genetics
Neoplasms, Experimental - microbiology
Retroviridae - genetics
Transfection
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Summary:We have used replication-competent retroviral vectors to express avian and murine ras genes in cultured chick embryo fibroblasts (CEF) and in chickens. Since the viral vectors are identical, it is possible to compare the oncogenic potential of the ras genes directly. The normal (12 gly) form of chicken c-Ha-ras is not oncogenic in vivo, nor does high-level expression transform CEF. Expression of murine v-ras or modified forms of chicken c-Ha-ras with either lysine or glutamine at position 12 transforms CEF and causes tumors in birds. However, the oncogenic potential of the transforming ras genes is different; the viruses that express the genes with lysine and glutamine at position 12 cause a distinct spectrum of tumors.
ISSN:0950-9232