RNA-mediated genetic transformation in Aspergillus nidulans

We developed a model-system for correcting genetic alterations of an Aspergillus nidulans strain (ribo, paba, bio, w, Acr) by treating protoplasts with total RNA extracted from another A. nidulans strain bearing wild type alleles for the same genetic markers. The results revealed the occurrence of a...

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Bibliographic Details
Published inCellular and molecular biology Vol. 35; no. 5; p. 573
Main Authors Zucchi, T M, Passos, Jr, G A, De Lucca, F L
Format Journal Article
LanguageEnglish
Published England 1989
Subjects
Aspergillus nidulans - genetics
Protoplasts
RNA, Fungal - genetics
Transformation, Genetic - genetics
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Summary:We developed a model-system for correcting genetic alterations of an Aspergillus nidulans strain (ribo, paba, bio, w, Acr) by treating protoplasts with total RNA extracted from another A. nidulans strain bearing wild type alleles for the same genetic markers. The results revealed the occurrence of a true genetic transformation. The phenomenon was RNA-dependent since it was abolished by pancreatic ribonuclease treatment. The term retrotransformation is proposed since the RNA messages artificially inserted into the mutant protoplast cells restore the wild type chromosomal information.