Cytogenetic analysis of BC2, a new human hepatoma cell line, by fluorescent in situ hybridization

Cytogenetic analysis of a new human hepatoma cell line BC2 was performed with conventional cytogenetic techniques and fluorescent in situ hybridization. Numerical and structural abnormalities were observed by conventional cytogenetics for chromosomes 1, 2, 4, 7, 8, 9, 10, 11, 15, 17 and 20. Chromoso...

Full description

Saved in:
Bibliographic Details
Published inAnnales de génétique Vol. 39; no. 3; p. 123
Main Authors Ingster, O, Hamon-Benais, C, Couturier-Turpin, M H, Guguen-Guillouzo, C, Lucas, J, Bernheim, A, Feldmann, G
Format Journal Article
LanguageEnglish
Published Netherlands 1996
Subjects
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - pathology
Chromosome Aberrations
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Liver Neoplasms - genetics
Liver Neoplasms - pathology
Male
Middle Aged
Tumor Cells, Cultured
Online AccessGet more information

Cover

More Information
Summary:Cytogenetic analysis of a new human hepatoma cell line BC2 was performed with conventional cytogenetic techniques and fluorescent in situ hybridization. Numerical and structural abnormalities were observed by conventional cytogenetics for chromosomes 1, 2, 4, 7, 8, 9, 10, 11, 15, 17 and 20. Chromosome painting allowed to specify the translocation of chromosome 1, and to characterize 3 markers from chromosome 8 and one marker from 9, which were unrecognizable by conventional techniques. Comparison of chromosome 1 abnormalities with those reported in the literature for other human hepatoma cell lines showed that structural abnormalities of chromosome 1 were present in different regions of this chromosome. A review of the literature was done, and the results discussed, suggesting that alterations of chromosome 1 may be important in hepatocarcinogenesis.
ISSN:0003-3995