A correlative study of in vivo and in vitro labeling index using bromodeoxyuridine in human brain tumors

The labeling index (LI) of 216 cases of human brain tumors was determined by the immunohistochemical technique with monoclonal antibody to bromodeoxyuridine (BrdU). The proliferative potential of 110 cases was estimated using the intra-operative intravenous infusion of BrdU at a dose of 200 mg/sq m....

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Bibliographic Details
Published inJournal of neuro-oncology Vol. 23; no. 3; p. 185
Main Authors Kharbanda, K, Dinda, A K, Sarkar, C, Karak, A K, Dhir, R, Mathur, M, Roy, S
Format Journal Article
LanguageEnglish
Published United States 01.01.1995
Subjects
Adolescent
Adult
Aged
Antibodies, Monoclonal
Brain Neoplasms - metabolism
Brain Neoplasms - pathology
Bromodeoxyuridine - metabolism
Cell Division - physiology
Child
Child, Preschool
Female
Humans
Hyperbaric Oxygenation
Immunohistochemistry
Infant
Male
Middle Aged
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Summary:The labeling index (LI) of 216 cases of human brain tumors was determined by the immunohistochemical technique with monoclonal antibody to bromodeoxyuridine (BrdU). The proliferative potential of 110 cases was estimated using the intra-operative intravenous infusion of BrdU at a dose of 200 mg/sq m. In another 106 cases, the in vitro technique of incubating freshly resected tumor tissue fragments with 100 microM bromodeoxyuridine was used. The BrdU LI in these tumors was then correlated with the histological types and the data as determined by both the in vivo and in vitro BrdU incorporation were compared. The results indicate that although in vivo and in vitro techniques could possibly provide equivalent data in some histologic types, a clear statistically valid proof however is not apparent from this study.
ISSN:0167-594X
DOI:10.1007/BF01059949