A mechanism for limiting reproduction influenza A virus reassortants with incomplete functional compatibility of hemagglutinin and neuraminidase gene products

• Published in: Voprosy virusologiĭ Vol. 43; no. 1; p. 17
• Main Authors: Kropotkina, E A, Rudneva, I A, Rusanova, O S, Varich, N L, Kadoshnikov, Iu P, Gambarian, A S, Kaverin, N V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.01.1998
• Subjects: Animals; Cell Line; Culture Media; Dogs; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - physiology; Influenza A virus - enzymology; Influenza A virus - genetics; Influenza A virus - pathogenicity; Influenza A virus - physiology; Kidney - ultrastructure; Kidney - virology; Microscopy, Electron; Neuraminidase - genetics; Neuraminidase - physiology; Virulence - genetics; Virus Replication - genetics
• ISSN: 0507-4088

The mechanism of decrease in the level of virus accumulation in reassortants with hemagglutinin (HA) and neuraminidase (NA) genes from different parents is studied. The reassortant viruses and their passage variants do not differ by the rate of virus protein production or their stability in infected cells. Electron microscopy and titration of infectious virus in culture fluid and cell-associated virus showed that the variants selected by serial passages accumulated mainly in the culture fluid, whereas the initial reassortant virions were predominantly cell-associated. These data suggest that incomplete removal of sialic acid residues by viral neuraminidase N1 in some reassortants results in re-attachment of virions to the infected cells and thus impairs the virus dissemination, which may be regarded as a reassortant-limiting factor probably significant for virus evolution.