A mechanism for limiting reproduction influenza A virus reassortants with incomplete functional compatibility of hemagglutinin and neuraminidase gene products

The mechanism of decrease in the level of virus accumulation in reassortants with hemagglutinin (HA) and neuraminidase (NA) genes from different parents is studied. The reassortant viruses and their passage variants do not differ by the rate of virus protein production or their stability in infected...

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Published inVoprosy virusologiĭ Vol. 43; no. 1; p. 17
Main Authors Kropotkina, E A, Rudneva, I A, Rusanova, O S, Varich, N L, Kadoshnikov, Iu P, Gambarian, A S, Kaverin, N V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.01.1998
Subjects
Animals
Cell Line
Culture Media
Dogs
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - physiology
Influenza A virus - enzymology
Influenza A virus - genetics
Influenza A virus - pathogenicity
Influenza A virus - physiology
Kidney - ultrastructure
Kidney - virology
Microscopy, Electron
Neuraminidase - genetics
Neuraminidase - physiology
Virulence - genetics
Virus Replication - genetics
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Summary:The mechanism of decrease in the level of virus accumulation in reassortants with hemagglutinin (HA) and neuraminidase (NA) genes from different parents is studied. The reassortant viruses and their passage variants do not differ by the rate of virus protein production or their stability in infected cells. Electron microscopy and titration of infectious virus in culture fluid and cell-associated virus showed that the variants selected by serial passages accumulated mainly in the culture fluid, whereas the initial reassortant virions were predominantly cell-associated. These data suggest that incomplete removal of sialic acid residues by viral neuraminidase N1 in some reassortants results in re-attachment of virions to the infected cells and thus impairs the virus dissemination, which may be regarded as a reassortant-limiting factor probably significant for virus evolution.
ISSN:0507-4088