Interleukin-4 production by the malignant cell line HUT78
Interleukin-4 (IL-4) is a cytokine difficult to induce in large quantities in freshly isolated cells. No malignant cell line has been described to date to produce IL-4 either constitutively or following activation. Here we report that HUT78 cells can be induced to produce IL-4 either by CD3 stimulat...
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Published in | Lymphokine and cytokine research Vol. 12; no. 1; p. 33 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.02.1993
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Subjects | |
Online Access | Get more information |
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Summary: | Interleukin-4 (IL-4) is a cytokine difficult to induce in large quantities in freshly isolated cells. No malignant cell line has been described to date to produce IL-4 either constitutively or following activation. Here we report that HUT78 cells can be induced to produce IL-4 either by CD3 stimulation under cross-linking conditions or by soluble CD3 mAbs in the presence of PMA. IL-4-specific mRNA could be demonstrated readily under such conditions. Using this experimental model we investigated the role of IL-2 for IL-4 production. The addition of IL-2 strongly costimulated CD3-induced IL-4 production, whereas blockade of the IL-2/IL-2R pathway reduced IL-4 production by approximately 50%. IL-2 alone, however, could not induce IL-4 production. These results indicate that IL-2 is a helper of cytokine necessary, but not sufficient, for IL-4 production in HUT78 cells. |
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ISSN: | 1056-5477 |